Lead Scientist, Center for Immuno-Oncology @ Dana-Farber Cancer Institute, Harvard Medical School
Senior Scientist @ Dana-Farber Cancer Institute, Center for Immuno-Oncology (CIO), Harvard University
Scientist I @ RaNA Therapeutics, Inc
Education:
About:
Technical skills:
Animal Handling: rodent surgery, murine hepatocyte isolation, genotyping and phenotyping, breeding, cells and macromolecules isolation, pulmonary function test (Flexivent), BAL, organ fixation and perfusion, external jugular catheterization, hysterectomy, in bloc organ removal, IV,IP, SC and IN drug administration, bleeding. histology skills.
Cell Culture: non-human primates, human and murine hepatocytes culture, primary and transformed cultures maintenance,
Technical skills:
Animal Handling: rodent surgery, murine hepatocyte isolation, genotyping and phenotyping, breeding, cells and macromolecules isolation, pulmonary function test (Flexivent), BAL, organ fixation and perfusion, external jugular catheterization, hysterectomy, in bloc organ removal, IV,IP, SC and IN drug administration, bleeding. histology skills.
Cell Culture: non-human primates, human and murine hepatocytes culture, primary and transformed cultures maintenance, passaging of cell lines, cloning, cryopreservation, stable cell line development, hybridoma formation and monoclonal antibody production, organ culture, plant tissue culture, cariotype, proliferation and apoptosis assays, mineral and protein transport analysis, flow cytometry (FACS), isolation and culture several primary cell types, stem cell culture.
Molecular Biology: PCR, rtPCR, bDNA, northern and southern blotting, agarose gel electrophoresis, DNA and RNA isolation, lipid-mediated transient transfection, adenovirus and lentivirus infection, plasmids purification, EMSA,
Pharmacology: drug screening, IC50 curves, in vivo efficacy studies, cell free and cell based binding and uptake assays.
Biochemistry and Immunology: western blotting, immunoprecipitation (IP) western, ELISA, electrophoresis, in vitro kinase assay, protein and mineral content analysis, spectrophotometry, spectrofluorometry.
General laboratory: buffers, cell culture media and solutions preparation, microscopy (light, phase contrast, fluorescence, imaging digital analysis), centrifugation, sterile technique, experiment design, supplies ordering, general maintenance, radioactive isotopes manipulation, animal and human tissue samples handling.
Computer: Macintosh and PC use, Microsoft Office (Word, Excel, Power Point), Adobe Photoshop, Optimas and Pro-image (Image digital Analyzer), Odyssey System, Prism, GraphPad, Diva and FlowJo, Canvas, bibliography searching.
Senior Scientist @ Center for Immuno-Oncology From April 2014 to Present (1 year 9 months) Boston, MassachusettsScientist I @ From April 2012 to April 2014 (2 years 1 month) cambridge, maPrincipal Research Associate @ From 2011 to 2012 (1 year) Sr. Research Associate @ From August 2008 to July 2011 (3 years) Research Associate @ From January 2008 to August 2008 (8 months) Research Fellow @ From April 2001 to January 2008 (6 years 10 months)
Biology @ Universidad Nacional de Córdoba From 1993 to 1999
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