Aroop Sircar's Email Addresses & Phone Numbers

Senior Scientist @ EMD Serono, Inc. Designer of novel immuno-oncology biologics: aPDL1-TGFb trap (M7824, Bintrafusp Alfa), high affinity SIRPa fusion protein such as anti-EGFR/SIRPa, bispecific antibodies.Domestic and international CRO outsourcing experience: cell line generation (lentivirus, crispr/cas9), immunohistochemistry, in vivo models, licensing of mouse and cell lines, and other immuno-oncology studies Billerica, MAScientist II @ EMD Serono, Inc....

Senior Scientist @ EMD Serono, Inc. Designer of novel immuno-oncology biologics: aPDL1-TGFb trap (M7824, Bintrafusp Alfa), high affinity SIRPa fusion protein such as anti-EGFR/SIRPa, bispecific antibodies.Domestic and international CRO outsourcing experience: cell line generation (lentivirus, crispr/cas9), immunohistochemistry, in vivo models, licensing of mouse and cell lines, and other immuno-oncology studies Billerica, MAScientist II @ EMD Serono, Inc. Designer of novel immuno-oncology biologics: aPDL1-TGFb trap, high affinity SIRPa fusion protein such as C225/SIRPa, bispecific antibodies.Domestic and international CRO outsourcing experience: cell line generation (lentivirus, crispr/cas9), immunohistochemistry, in vivo models, licensing of mouse and cell lines, and other immuno-oncology studies From May 2016 to June 2018 (2 years 2 months) Billerica, MAScientist ImmunoTechnologies @ EMD Serono, Inc. • Drive protein modeling strategy for Immuno-Oncology programs to meet project goals• Develop bioinformatics strategies for developing new molecular entities with novel immune mechanism of action• Computational structural and bioinformatics support: ortholog analysis, protein modeling/design, humanization, epitope analysis, linker design, structural analysis of related molecules to determine the possibility of cross/dual reactivity• Patent application filing for new biological entities• Patent review to determine lead candidates for synthesis of control molecules • Provide support for preparation of computationally relevant slides for new target proposal presentations• Strategic outsourcing to forge long term partnerships for supporting pipeline projects• Select preferred vendors by comparing quotations for multiple CROs and assessment of top candidates by performance on pilot projects• Identify technically competent Contract Research Organizations (CROs) to bridge gaps in areas where no in-house capabilities exist• Coordinate outsourced projects: Confidentiality Disclosure Agreement execution, technical assessment and finalization of Statement of Work, serve as a liaison between in-house and CRO scientists to troubleshoot, ensure deliverables meet timelines• Ever expanding outsourced projects span gene->protein, cell line development, immunohistochemistry, antibody screening campaigns, protein sequencing, hybridoma sequencing, protein expression scouting From May 2014 to May 2016 (2 years 1 month) Billerica, MAPost Doctoral Scientist - Immuno-Oncology @ EMD Serono, Inc. • Computational Antibody Humanization & Murinazation• Computational Antibody Affinity Maturation• Design antibody fusion proteins and relevant linkers• Create work plans for DNA synthesis (Vector NTI), subcloning and expression• Evaluate Contract Research Organizations (CROs)• CRO based project management From March 2013 to May 2014 (1 year 3 months) Billerica, MAPost Doctoral Scientist - Antibody Design @ EMD Serono, Inc. • Enhance antibody stability by computational mutagenesis (compute ΔΔGbinding by RosettaInterface)→Enhance expression• Enhance antibody binding (affinity maturation) by computational mutagenesis (protein design using RosettaInterface) • Incorporate peptides into antibody framework & modeling FC fusion proteins by developing customized algorithms• Outsource gene synthesis, protein expression and purification by working with CROs (quotations from multiple vendors, explore new vendors)• Design experiments to test computational models• Improve understanding and computational design based on experimental feedback (Surface Plasmon Resonance - Biacore, ELISA, FACS, Mass Spectrometry) From August 2010 to March 2013 (2 years 8 months) Billerica, MAGraduate Student @ Johns Hopkins University • Protein structure prediction: Novel computational protocol for antibody homology modeling with CDR H3 loop modeling & optimization of heavy & light chain orientation, RosettaAntibody. • Induced fit docking: Algorithms for prediction of interaction complex of antigen & homology modeled antibody with paratope optimization: Monte Carlo with minimization, SnugDock.• Structural bioinformatics: Sequence alignment based structural classification & homology modeling of camelid antibodies (VHH). (Sponsored by UCB S.A.)• Use of experimental NMR data: Prediction of ternary complex of K48-linked di Ubiquitin & E2G2 enzyme by incorporating NMR CSP & PRE data. (with Joel Tolman)• Protein-protein complexes: Successful predictions in world-wide challenge CAPRI 6-19• Web server for antibody homology modeling : RosettaAntibody From September 2004 to August 2010 (6 years) Baltimore, Maryland AreaSummer Intern @ University of Cambridge Advisor: Dr. Kenji Mizuguchi, (Wellcome Trust Research Career Development Fellow)Crystallography and Biocomputing, Dept. of BiochemistryProject: Structural assignment for the Drosophila genome and mapping of known mutationsThe work comprised of bioinformatics involving high throughput modeling and automatic analysis of results to generate useful statistics. The programming was mainly carried out in Perl and Python, with MySQL being used to store the data.Short Term Courses: 1.Introductory Bioinformatics Course conducted by United Kingdom Human Genome Mapping Project Resource Center at the Department of Genetics, University of Cambridge, UK2. Sequencing Project Management Using the Staden Package conducted by United Kingdom Human Genome Mapping Project Resource Center at the Department of Genetics, University of Cambridge, UK From May 2003 to July 2003 (3 months) Cambridge, United KingdomSummer Intern @ National Center for Biological Sciences (NCBS) Advisors: Dr. Satyajit Mayor and Dr. R. SowdhaminiProject: Delving Deep into Deep Orange and Carnation from a Computational ViewpointThe work involved modeling (using MODELLER) of the two Drosophila proteins Deep Orange and Carnation and finally docking them to find out the interacting regions (using GRAMM). From May 2002 to July 2002 (3 months) Bengaluru Area, IndiaVisiting Summer Research Program Student @ Tata Institute of Fundamental Research (TIFR) Advisor: Prof.K.S.KrishnanProjects:1) An attempt to find genes responsible for synaptic transmission using Drosophila melanogaster as a model system.The work involved extensive Fly Pushing and screening flies for phototactic and paralytic defects.2) Effect of Conus toxins on Drosophila melanogaster From May 2001 to July 2001 (3 months) Mumbai Area, India