Highly motivated life science professional with scientific expertise, leadership, management and technical skills. An excellent team player with multidisciplinary background integrated in industrial experience of 2+ years in industrial microbiology, molecular biology and biotechnology
Technical proficiency consists of a PhD in Biotechnology (specialization-molecular biology) (2012), Masters in Biotechnology (2005) and Bachelors in Industrial Microbiology Sciences (2003). Published over 6 research papers and participated in various National and International conferences with 6 years of supervising and mentoring experience.
Principal Scientist (R&D Molecular Biology) @ Engaged in the Research and Development Department of the company.
• Metabolic engineering in small molecules (streptomyces) and screening (shake flask and 5 L fermenter) for enhanced production of various molecules (Tacrolimus, Lipstatin, Sirolimus)
• Genetic modifications in Pichia host for enhanced production of Biosimilars
• Screening of generated mutants based on HPLC analysis for the estimation of desired metabolites
• Generation of c-DNA library from Penicillium host for detecting various genes
• Experience in molecular biology of mammalian cell culture (MAbs)
• Experience in matrix-managed work teams and audited different labs being a part of auditing team
• Responsible for the Environmental Safety (EHS) and QMS activities in the lab
• Expertise in documentation Systems (Lab Notebooks, SOPs, GMs, EHS, Process Validation), data collection, processing, preparation and presentation of Technical Reports, Technology data transfer and Development Reports Expense Budgets, Work Orders and Purchasing Systems
• Responsible for following cGMP/GLP and ICH guidelines and Process Validation
• Trained employees and trainees on molecular biology techniques From November 2012 to Present (3 years 2 months) PhD in Biotechnology @ 6 yrs of research experience during (2006-2012) PhD Thesis Focus: “Development of Metabolic and molecular insights on Limonin biotransformation by Pseudomonas putida”
Abstract
• Physiological characterization of Pseudomonas putida PpG7 in biotransformation of limonin
• Localization, purification and Identification of the unknown enzyme(s) involved in the limonin biotransformation by P.putida G7
• Genetic analysis of lim- Tn5 mutants and identification of the unknown gene(s) by cloning and sequencing that are responsible for limonin biotransformation
• Development and optimization of a bioreactor using P.putida G7 for an industrial application From February 2006 to April 2012 (6 years 3 months) Senior research fellow @ 2 yrs of research experience as a CSIR (Senior Research Fellow) during April 2009- March 2011
• Bacteriological analysis of food products using AOAC methodology
• Tests include: Aseptic techniques, testing for Pathogens like Staphylococcus aureus, Listeria monocy togenes, Salmonella, Clostridium, Bacillus cereus, Lactobacilli and E. coli 0157
• Water Portability Testing on both pool and raw water samples
• The use of Petri film, MPN and pour plate methods for bacterial identification
• Media optimization for the adherent cell lines From April 2009 to March 2011 (2 years) Teaching Assistant @ Worked as a Teaching Assistant, in Department of Biotechnology & Environmental Sciences at Thapar University, Patiala
• Mentored Master students (M.Sc, M.Tech) for their dissertations in the Lab
• Responsible for conducting tutorials and practicals for BE (Bachelor of Engineering) students
• Responsible for giving exam invigilation duties and completion of departmental official work From July 2007 to June 2008 (1 year)
PhD, Biotechnology @ Thapar University, Patiala, Punjab, India From 2006 to 2012 Master of Science (MSc), Biotechnology @ Chaudhary Charan Singh University From 2003 to 2005 B.Sc, Industrial Microbiology @ Kurukshetra University From 2000 to 2003 10+2, Physics, Chemistry, Biology, English, Hindi @ Seventh Day Adventist Inter College, Roorkee, UP, India From 1997 to 1999 10th @ St. Francis School, Shamli, UP, India From 1986 to 1997 Meenakshi Malik, PhD is skilled in: Molecular Biology, Biotechnology, Proteomics, Protein Chemistry, Biochemistry, Protein Purification, Protein Expression, Cell Culture, Immunology, ELISA, PCR, Bioinformatics, Lifesciences, HPLC, Microbiology